Dear Angshu The answer to your question requires to define precisely the term of "stoichiometry". *If you consider the hexamer as "the molecule B", then the expected stoichiometric ratio is 1/1 (one molecule A should bind to 1 hexamer B). *But if you consider the monomer of B as "the molecule" in the cell, then the expected stoichiometric ratio is 1/6 (one molecule of A should bind to 6 monomers of the hexamer B). Accordingly, you have to define the concentration in the cell as follows: if you consider the hexamer as "the molecule B" and, let's suppose you have [B] = 10 µM of hexamer, then [B] = 60 µM if you consider the monomer of B as "the molecule". I hope I answered your question. Philippe Dumas
De: "Angshu Dutta" <angshu.cryst...@gmail.com> À: "CCP4BB" <CCP4BB@JISCMAIL.AC.UK> Envoyé: Vendredi 21 Février 2020 15:24:53 Objet: [ccp4bb] ITC Stoichiometry Dear all, Apologies for an off-topic question. There are two proteins- A(monomer) and B(hexamer). As per reports, one molecule of A(monomer) should bind to one molecule of B(hexamer). In order to show the interaction between the two proteins through ITC, A is taken in the syringe and B is taken in the cell. What kind of stoichiometry values should be expected? I look forward to your responses. Many thanks in advance. Best, Angshu To unsubscribe from the CCP4BB list, click the following link: [ https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 | https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ] ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
