Dear Vito, Could you precise in what kind of maps are you experiencing these effects: 2FoFc refinement maps or experimental phasing ? I had this kind of effects long ago in experiemental phasing due to Fourier transform ripple effects. This could be due to scaling problems, low resolution truncation or incompletness (maybe intensity overloads). Another source condition could be local X-ray dose degradation due to the high absoption of the metals. Best regards, Pierre Legrand PROXIMA-1, SOLEIL ________________________________________ De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Vito Calderone [calder...@cerm.unifi.it] Envoyé : mercredi 10 juin 2020 08:42 À : CCP4BB@JISCMAIL.AC.UK Objet : [ccp4bb] Heavy atom vs light atoms density
Dear All, many of us have probably experienced that, in the diffraction of protein ligands containing heavy atoms (cisPt, I3C, etc), the overwhelming electron density of the metal can totally flatten that of the light atoms around (or rather make it look insignificant). Is anyone aware of an article/review (to use as a reference) in which this is clearly stated/pointed out? Best regards Vito Calderone ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/