Dear Vito, We accumulated quite a good deal of knowledge of this type of complex, firstly the platins, then later, led by Dr Alice Brink, rhenium complexes with proteins. This review describes some of this:- https://pubs.rsc.org/en/content/articlelanding/2017/mt/c7mt00219j#!divAbstract The flattening of electron density that you refer to was not an issue but does become a problem though with uranyl hydrides (not involving proteins to my knowledge), solved by using neutrons, work led by the late Prof Bob Bau. Exchange of the ligands in the often quite complex biochemical mix of a protein crystal can be more challenging to decipher however. Chlorine versus ammine in platins can be distinguished with high enough resolution data but confounded at lower resolution. Sharing of raw diffraction data has proved very informative and by sharing all of our raw data in the platins new improved details have been squeezed out, with Prof Kay Diederichs leading us and also through Dr Ivan Shabalin and colleagues‘ helpful and expert work, in a very broad study, we learnt more about our platins and stimulated us in yet more detailed analyses with Dr Loes Kroon-Batenburg and Dr Toine Schreurs in Utrecht. It is all a most interesting topic especially given the wide medicinal roles of these heavy metals which “must bind somewhere in us”, and not only used as tools for solving our phase problem! Best wishes, John Emeritus Professor John R Helliwell DSc
> On 10 Jun 2020, at 07:42, Vito Calderone <calder...@cerm.it> wrote: > > > Dear All, > many of us have probably experienced that, in the diffraction > of protein ligands containing heavy atoms (cisPt, I3C, etc), the overwhelming > electron density of the metal can totally flatten that of the light atoms > around (or rather make it look insignificant). > Is anyone aware of an article/review (to use as a reference) in which this is > clearly stated/pointed out? > Best regards > > Vito Calderone > > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
