Correction: It should read "with the detector almost but not quite hitting the source."
On 02.07.20 17:13, Navdeep Sidhu wrote: > Alexander Blake wrote a nice chapter on small-molecule crystallization > in this book, if you run into problems in the crystallization stage: > > Alexander Blake. Crystal growth and evaluation (Chapter 3). In Clegg, > William (Ed.) Crystal Structure Analysis: Principles and Practice. 2nd > Edition. IUCr/Oxford, 2009 > <https://www.oxfordscholarship.com/view/10.1093/acprof:oso/9780199219469.001.0001/acprof-9780199219469>; > <https://global.oup.com/academic/product/crystal-structure-analysis-9780199219476>. > > With the detector almost but not quite hitting the detector, one can > typically collect all the data one needs on a home Cu source. Overloads > can often be a problem---as has been pointed out out for > synchrotrons---even on a home source. But they can usually be dealt with > easily by modifying exposure, collecting lower- and higher-angle data > separately, leaving some overlap between the two regions (see "Intensity > level", Blake, Ch. 6.7.1, p. 88 in book above) and scaling. In extreme > cases, one may need to lower the generator power. > > The nice thing about the slow pace of data collection on a home source > is that one can think while doing the collection, thus catching and > correcting most problems before it's too late. In Ch. 7.6 in the book, > Blake also discusses some other problem cases. (The whole book's a great > read.) > > Cheers, > Navdeep > > > >>>> --- >>>> On 01.06.20 23:50, Jiyuan Ke wrote: >>>> > Hi Everyone, >>>> > >>>> > I want to crystallize a small organic molecule. I have >>>> very limited >>>> > experience in small molecule crystallography. I found >>>> that the Crystal >>>> > Screen HT from the Hampton research is good for both >>>> small molecule and >>>> > macromolecule crystallization. Plan to set up a sitting >>>> drop screen just >>>> > like setting up protein crystallization. I don’t know >>>> if this is the >>>> > proper way to do it. Is the MRC sitting drop 2-well >>>> plate (HR3-083) used >>>> > for protein crystallization good for small molecule >>>> crystallization? Are >>>> > there any special plates used for small molecule >>>> crystallization? Is >>>> > room temperature ok or not? >>>> > >>>> > For data collection, can I use the beamline for protein >>>> crystals to >>>> > collect data on small molecule crystals? Larger >>>> oscillation angle, >>>> > shorter exposure, reduced beam intensity? >>>> > >>>> > For structure determination, is SHELXL the preferred >>>> software for >>>> > solving small molecule structures? >>>> > >>>> > If anyone has experience in small molecule >>>> crystallography, please >>>> > help. Thanks! >>>> > >>>> > Best Regards, >>>> > >>>> > -- >>>> > >>>> > *Jiyuan Ke, Ph.D.* >>>> > >>>> > * >>>> > * >>>> > >>>> > Research Investigator >>>> > >>>> > H3 Biomedicine Inc. >>>> > >>>> > 300 Technology Square, Floor 5 >>>> > >>>> > Cambridge, MA 02139 >>>> > >>>> > Phone: 617-252-3923 >>>> > >>>> > Email: jiyuan...@h3biomedicine.com >>>> <mailto:jiyuan...@h3biomedicine.com> >>>> <mailto:jiyuan...@h3biomedicine.com >>>> <mailto:jiyuan...@h3biomedicine.com>> >>>> > >>>> > Website: www.h3biomedicine.com >>>> <http://www.h3biomedicine.com/> >>>> <http://www.h3biomedicine.com/> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
