Dear Christian, We occasionally observe binding to only one monomer of a multimeric complex. I don’t think this invalidates the biological significance of your finding. I would superimpose the different monomers to see if they have (slightly) different conformations that prevent ligand binding. Also for cocrystallization experiments, the presence of or absence of channels is irrelevant. This only matters for soaking experiments.
Best, Herman Von: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> Im Auftrag von Christian GALICIA Gesendet: Dienstag, 27. Oktober 2020 11:19 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] ligand bound to only one chain in the crystal Hello, In our structure only one chain in a crystallographic trimer (non-biological) shows a ligand bound to it (with clear density). There doesn't seem to be any channels (or lack of them) favoring that specific site. Can the community give your opinion on whether this can make the presence of the ligand or its biological role questionable, and give any examples of similar cases you might be aware of. Thank you. -- Christian Galicia Post Doctoral Scientist E-mail: cgali...@vub.be<mailto:cgali...@vub.be> ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1<https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2FWA-JISC.exe%3FSUBED1%3DCCP4BB%26A%3D1&data=04%7C01%7CHerman.Schreuder%40SANOFI.COM%7C72eeddf60d424935fe4f08d87a6335ca%7Caca3c8d6aa714e1aa10e03572fc58c0b%7C0%7C0%7C637393914241788376%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=DRdXPV6zc%2BbKfYNk7sVxqeAxhOEZ9bgKKdtWhpFVfYw%3D&reserved=0> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
