Dear Saurabh, Your crystals are gorgeous!
Which cryoprotectant did you use? Best, Everton. Sent from my iPhone > On Mar 21, 2021, at 11:04 AM, Saurabh Upadhyay <saurabh807...@gmail.com> > wrote: > > > Dear All, > > I am trying to crystalize a protein, which occurs in monomeric (60kDa) and > tetrameric (240kDa) form. The protein during crystallization was in MES > buffer pH-6 and the method of crystallization was "Sitting drop" in "Proplex" > condition. Some of the conditions in which crystals were obtained are: > > 1) 0.1M HEPES pH-7; 10% (w/v) PEG 4000 > 2) 0.1M Tris, pH-8; 8% (w/v) PEG 8000 > 3) 0.1M Tris, pH-8; 25% (v/v) PEG 400 > > For reference, I have attached the images of the crystals observed below. > > However, the diffraction pattern obtained and the resolution of crystals were > very poor. Even some mosaicity has been observed. > > Kindly suggest some methods or modifications, to improve the resolution and > diffraction pattern of the crystals obtained. > > Thanking You, > Sincerely, > Saurabh Upadhyay, > Ph.D. Scholar > c/o Dr. Ashok kumar Patel, > Kusuma School of Biological Sciences, > Indian Institute of Technology, Hauz Khas, > New Delhi-110016, India > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > <Crystals by Sitting Drop.pptx> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
