Definitely water pentamer, no doubt at all ;-0 Cheers, Jon.C. Sent from ProtonMail mobile
-------- Original Message -------- On 22 Mar 2021, 14:16, Mark J. van Raaij wrote: > The ring looks too big to be imidazole or a nucleotide or a carbohydrate, so > it’s probably mainly water molecules. > Perhaps partially replaced by PEG to explain the density between them (i.e. > water molecules in most copies of the protein and PEG in some other copies). > I’ve seen horse-shoe shaped PEG in a high-res structure before, PEGs in > several confirmations might explain a circle. > Practically speaking, I’d first model five waters and see if they refine well. > > Mark > >> On 22 Mar 2021, at 14:58, Sam Tang <samtys0...@gmail.com> wrote: >> >> Hello fellow colleagues >> >> Hope you are all well while the pandemics persists. I just wonder if anyone >> may have an idea what this density (looking like a pentagon) might be. The >> data was collected to 1.8 A and crystal was grown in Bis-tris + PEG3350. >> Imidazole residual? Nucleotide (the protein itself is nucleotide-binding, >> but shouldn't be at this particular site)? >> >> https://drive.google.com/file/d/1L9UBFmW72P214itM2HJR_DVy3FaA6FEZ/view?usp=sharing >> >> Thanks! >> >> BRS >> >> Sam >> >> --------------------------------------------------------------- >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > --------------------------------------------------------------- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
