Dear CCP4 community,
sorry for the off topic question but I am struggling with enzymatic
assays and I would need your help.
I am trying to measure dephosphorylation of a phosphorylated kinase by a
ser/thr phosphatase. So far I tried to measure it by malachite green
assay, anti pSer/pThr antibody and PNPase coupled assay but nothing
really worked for me. NMR is unfortunately not an option since I can't
make enough of the proteins.
So any hints on phosphatase assays would be helpful.
Thanks!
Best wishes,
Andrea
--
Andrea Moretti
Structural Plant Biology Laboratory
Department of Botany and Plant Biology
Sciences III
University of Geneva
30 Quai E. Ansermet
1211 Geneva
Switzerland
Email: andrea.more...@unige.ch
Phone: +41 22 379 3029 (office)
web:http://structplantbio.org
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