Dear all, Thanks a lot for all your nice suggestions, I really appreciate them.
I am now digging through all the methods you suggest trying to find the one that will solve my enzimatic issue. For the ones that asked what was not working, I am trying to replicate some data but with not much success until now. Thanks again for your help and have a nice day. Andrea > Il giorno 16 set 2021, alle ore 17:11, Schreuder, Herman /DE > <herman.schreu...@sanofi.com> ha scritto: > > > It may also be possible to separate the different phosphorylated forms on an > isoelectric focusing gel. > Best, > Herman > > Von: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> Im Auftrag von Patrick Loll > Gesendet: Mittwoch, 15. September 2021 15:12 > An: CCP4BB@JISCMAIL.AC.UK > Betreff: Re: [ccp4bb] Phosphatase enzymatic assay > > Phos-tag gels are an option (albeit a fiddly one). > Cheers, > Pat Loll > > > ___________________________ > > Patrick J. Loll, PhD (he, him, his) > Professor of Biochemistry & Molecular Biology > Drexel University College of Medicine > Room 10-102 New College Building > 245 N. 15th St. > Philadelphia, PA 19102-1192 USA > > (267) 359-2653 > pj...@drexel.edu > > > > On 15 Sep 2021, at 8:28 AM, Andrea Moretti <andrea.more...@unige.ch> wrote: > > Dear CCP4 community, > > sorry for the off topic question but I am struggling with enzymatic assays > and I would need your help. > > I am trying to measure dephosphorylation of a phosphorylated kinase by a > ser/thr phosphatase. So far I tried to measure it by malachite green assay, > anti pSer/pThr antibody and PNPase coupled assay but nothing really worked > for me. NMR is unfortunately not an option since I can't make enough of the > proteins. > > So any hints on phosphatase assays would be helpful. > Thanks! > > Best wishes, > Andrea > > > -- > Andrea Moretti > Structural Plant Biology Laboratory > Department of Botany and Plant Biology > Sciences III > University of Geneva > 30 Quai E. Ansermet > 1211 Geneva > Switzerland > Email: andrea.more...@unige.ch > Phone: +41 22 379 3029 (office) > web:http://structplantbio.org > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing > list hosted by www.jiscmail.ac.uk, terms & conditions are available at > https://www.jiscmail.ac.uk/policyandsecurity/ > > Patrick Loll > pjl...@gmail.com > > > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
