Hi , 1) If you are collecting the data at the APS the samples frozen in propane can be legally shipped there (check APS web site for information) 2) The samples CANNOT be mounted using either the ACTOR or ALS Style auto mounter. But the samples can be shipped using either the Rigaku pucks or the Uni-pucks with a vial cover adapter. In any case you would need to manually mount and collect data or have the samples retrieved after the propane melted with the robotic mounter and mounted later by the robot for data collection.
>From my extensive experience quenching samples using propane it has been >possible to freeze ALL in LN2 after a) perfecting cryoprotectant, b) >perfecting freezing technique, c) modifying crystal growth conditions. Steve Ginell > On Jan 26, 2022, at 1:31 PM, Joseph Ferrara <joseph.ferr...@rigaku.com> wrote: > > When using Xe derivatization for SAD phasing, we (mostly Jim Pflugrath) used > carbon tetrafluoride. CF4 has a melting point of 89.5K, just four degrees > warmer than propane and is not flammable. > > Joe Ferrara > > -----Original Message----- > From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of James Holton > Sent: Wednesday, January 26, 2022 13:07 > To: CCP4BB@JISCMAIL.AC.UK > Subject: [EXTERNAL] Re: [ccp4bb] freezing in ethane / propane - unipucks ? > > I think the issue with propane at at least some light sources is that it is > flammable. Makes shipping and safety more complicated. > > How hard would it be to let the propane melt off in a cryo gas stream at your > home lab? Then use tongs to transfer the pin into liquid N2 for handling as > usual? If you don't have a working N2 gas stream and are on a budget they > are not that hard to build: > https://doi.org/10.1107/S0021889894006357 > > Cheers, > > -James Holton > MAD Scientist > > > On 1/26/2022 9:25 AM, Guenter Fritz wrote: >> Dear Dom, >> >> thanks a lot. Yes, this might work sending a combipuck alongside with >> a good bottle for the local contact. >> I was wondering whether the grippers can handle a block of propane? >> >> Best wishes, >> guenter >>> Dear Guenter, >>> >>> Would the use of vials inside combi-pucks >>> (https://www.mitegen.com/product/combipuck-system/) and some >>> arrangements with your local contact at the other end, perhaps help >>> with using propane remotely? >>> >>> BW, >>> >>> D >>> >>> On 26/01/2022 16:53, Guenter Fritz wrote: >>>> Dear all, >>>> >>>> we have some delicate crystals which might benefit from freezing in >>>> propane. In former times (when I was still travelling to the >>>> beamlines) we waited until the propane was solid in the vial and >>>> then let the propane thaw in the cryo stream at the beamline. >>>> >>>> But how can we do this in these days with unipucks and no manual >>>> mounting? >>>> >>>> I was thinking about freezing in ethane and then transfer the loops >>>> to liquid nitrogen, similarly we handle grids for cryo EM. Does >>>> somebody has tried that? Any experience, tips & tricks would be very >>>> welcome! Thanks in advance and best regards, >>>> >>>> Guenter >>>> >>>> #################################################################### >>>> #### >>>> >>>> >>>> To unsubscribe from the CCP4BB list, click the following link: >>>> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >>>> >>>> This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a >>>> mailing list hosted by www.jiscmail.ac.uk, terms & conditions are >>>> available at https://www.jiscmail.ac.uk/policyandsecurity/ >>> >> >> ###################################################################### >> ## >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> >> This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a >> mailing list hosted by www.jiscmail.ac.uk, terms & conditions are >> available at https://www.jiscmail.ac.uk/policyandsecurity/ > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing > list hosted by www.jiscmail.ac.uk, terms & conditions are available at > https://www.jiscmail.ac.uk/policyandsecurity/ > > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing > list hosted by www.jiscmail.ac.uk, terms & conditions are available at > https://www.jiscmail.ac.uk/policyandsecurity/ ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
