Hi Steve,
Hi ,
1) If you are collecting the data at the APS the samples frozen in propane can
be legally shipped there (check APS web site for information)
2) The samples CANNOT be mounted using either the ACTOR or ALS Style auto
mounter. But the samples can be shipped using either the Rigaku pucks or the
Uni-pucks with a vial cover adapter. In any case you would need to manually
mount and collect data or have the samples retrieved after the propane melted
with the robotic mounter and mounted later by the robot for data collection.
I am in Europe, so I would go for ESRF / SLS.
From my extensive experience quenching samples using propane it has been
possible to freeze ALL in LN2 after a) perfecting cryoprotectant, b) perfecting
freezing technique, c) modifying crystal growth conditions.
:)) yes better crystals is always the best solution. But we are happy
that we have crystals at all after some efforts (insect cells, low
solubility, crystallization in the glove box, ....)
Cheers Guenter
Steve Ginell
On Jan 26, 2022, at 1:31 PM, Joseph Ferrara <joseph.ferr...@rigaku.com> wrote:
When using Xe derivatization for SAD phasing, we (mostly Jim Pflugrath) used
carbon tetrafluoride. CF4 has a melting point of 89.5K, just four degrees
warmer than propane and is not flammable.
Joe Ferrara
-----Original Message-----
From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of James Holton
Sent: Wednesday, January 26, 2022 13:07
To: CCP4BB@JISCMAIL.AC.UK
Subject: [EXTERNAL] Re: [ccp4bb] freezing in ethane / propane - unipucks ?
I think the issue with propane at at least some light sources is that it is
flammable. Makes shipping and safety more complicated.
How hard would it be to let the propane melt off in a cryo gas stream at your
home lab? Then use tongs to transfer the pin into liquid N2 for handling as
usual? If you don't have a working N2 gas stream and are on a budget they are
not that hard to build:
https://doi.org/10.1107/S0021889894006357
Cheers,
-James Holton
MAD Scientist
On 1/26/2022 9:25 AM, Guenter Fritz wrote:
Dear Dom,
thanks a lot. Yes, this might work sending a combipuck alongside with
a good bottle for the local contact.
I was wondering whether the grippers can handle a block of propane?
Best wishes,
guenter
Dear Guenter,
Would the use of vials inside combi-pucks
(https://www.mitegen.com/product/combipuck-system/) and some
arrangements with your local contact at the other end, perhaps help
with using propane remotely?
BW,
D
On 26/01/2022 16:53, Guenter Fritz wrote:
Dear all,
we have some delicate crystals which might benefit from freezing in
propane. In former times (when I was still travelling to the
beamlines) we waited until the propane was solid in the vial and
then let the propane thaw in the cryo stream at the beamline.
But how can we do this in these days with unipucks and no manual
mounting?
I was thinking about freezing in ethane and then transfer the loops
to liquid nitrogen, similarly we handle grids for cryo EM. Does
somebody has tried that? Any experience, tips & tricks would be very
welcome! Thanks in advance and best regards,
Guenter
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