Hi Firdous, When I have used ADP/AF3 as a transition state analogue in crystallography, I have always formed the ADP/AF3 complex in place by adding ADP, aluminum nitrate, and fluoride at a 1:1:6 ratio to my protein. I then used that mixture directly for crystallogenesis.
Best, Michael From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of Firdous Tarique Sent: Tuesday, June 21, 2022 12:38 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Solubility of Aluminum Fluoride EXTERNAL MAIL Hi Sorry for the off topic question but can anybody please suggest how to dissolve Aluminium Fluoride in water ? I know it has little solubility in water but have seen people using AF3 for their structural studies (ADP + AF3 as ATP analogue). Is there any other solvent where they have made AF3 solution or is there some trick to dissolve it in water?. Thanks Firdous ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 CAUTION: This email originated from outside UTSW. Please be cautious of links or attachments, and validate the sender's email address before replying. ________________________________ UT Southwestern Medical Center The future of medicine, today. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
