Sorry, I should have said ADPBeF3 (not ADPBeF4), and increase the NaF
proportion for ADPAlF4. Which in practice means reducing the concentrations of
everything else due to limited solubility of NaF.
From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Dr. Kevin M Jude
<kj...@stanford.edu>
Date: Wednesday, June 22, 2022 at 9:10 AM
To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK>
Subject: Re: [ccp4bb] Solubility of Aluminum Fluoride
I posted the following to ccp4bb in 2017 in response to a similar question
about ADPBeF4. For AlF4 you’ll increase the NaF stoichiometrically:
reagent [stock] (mM) final concentration (mM) volume (µl)
BeCl2 1000 90 9
NaF 750 450 60
ADP, pH 8 100 30 30
H2O 1
total volume 100
To prepare ADP:Be:F (1:3:15)
Prepare BeCl2 stock in the fume hood due to release of Cl2 gas
thoroughly mix BeCl2 and NaF
Add ADP
dilute with H2O to final volume
This is done at room temperature and the reactions are fast. Careful mixing
(and adjusting the pH of the ADP beforehand) are important because the BeCl2
stock will precipitate at neutral or elevated pH, and the BeFx solution is
quite acidic.
From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Firdous Tarique
<kahkashantari...@gmail.com>
Date: Tuesday, June 21, 2022 at 5:13 PM
To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK>
Subject: Re: [ccp4bb] Solubility of Aluminum Fluoride
Thanks for your input. To avoid precipitation what about doing something like
this? For example, as we know: AlCl3 + 3NaF = AlF3 + 3NaCl
Therefore, adding 100mM AlCl3 plus 300mM NaF to get 100mM of AlF3 solution,
then directly using this 100 mM stock of AlF3 for the final dilution, say 1 or
2 mM in the reaction mix ? Or it is recommended to use AlCl3 and NaF hoping to
form AlF3 in the reaction mix.
Best
Faisal
On Tue, Jun 21, 2022 at 2:08 PM Guillaume Gaullier
<guillaume.gaull...@univ-tlse3.fr<mailto:guillaume.gaull...@univ-tlse3.fr>>
wrote:
Hi Firdous,
I have read a fair amount of papers about ATP-dependent chromatin remodelers in
which ADP-BeF3 is often cited. It is made as follows (quoting from one of these
papers): "ADP-BeF3 was prepared as a 10x stock (10 mM ADP, 30 mM BeSO4, 150 mM
NaF, 10 mM MgCl2), freshly before use."
So the trick is to get all you need from a mixture of soluble compounds,
instead of from only one poorly soluble salt. Not sure this will work the same
with Al instead of Be, but it’s easy enough to try: use any known-soluble salt
of Al instead of this BeSO4, and see if it works. NaF is extremely toxic, so
use adequate protective measures (PPE, hood, etc.).
Good luck,
Guillaume
On 21 Jun 2022, at 19:38, Firdous Tarique
<kahkashantari...@gmail.com<mailto:kahkashantari...@gmail.com>> wrote:
Hi
Sorry for the off topic question but can anybody please suggest how to dissolve
Aluminium Fluoride in water ? I know it has little solubility in water but have
seen people using AF3 for their structural studies (ADP + AF3 as ATP analogue).
Is there any other solvent where they have made AF3 solution or is there some
trick to dissolve it in water?.
Thanks
Firdous
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