If you know at least something about your protein, organism, type of molecule, ..., you could try mass spectrometry peptide mapping to known sequences, this may give you some answers for the ambiguities you might be seeing, if nothing else ..
Jan On Fri, Jul 29, 2022 at 12:15 PM Jon Cooper < 0000488a26d62010-dmarc-requ...@jiscmail.ac.uk> wrote: > Hello, I am looking for suggestions of ways to check a 1.7 Angstrom X-ray > sequence for a protein where it is impractical to do experimental > sequencing, protein or DNA. The structure refines to publishable R/R-free > and the main ambiguities seem to be Thr/Val, Asp/Asn and Glu/Gln where > alternative H-bonding networks are possible. Running alpha-fold seems an > interesting option? Any suggestions much appreciated. > > Cheers, Jon.C. > > Sent from ProtonMail mobile > > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > -- Jan Dohnalek, Ph.D Institute of Biotechnology Academy of Sciences of the Czech Republic Biocev Prumyslova 595 252 50 Vestec near Prague Czech Republic Tel. +420 325 873 758 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
