Hi Kavya, Try https://csb.wfu.edu/tools/vmcalc/vm.html
This tells you that a 30kD protein simply does not fit the cell. I am pretty sure you crystallised the ligand, or TCEP actually. Also, if you look at the diffractions pattern, its clear the crystal diffracts beyond 1.0A, diffraction spots are really very very very strong at 2.0A. On 3 Feb 2023, at 09:22, kavyashreem <kavyashr...@instem.res.in<mailto:kavyashr...@instem.res.in>> wrote: Dear all, We crystallized a protein (30kDa) + ligand (by cocrystallization), in the condition 10%PEG3350, 50mM Zinc acetate. Protein was in the buffer 20mM HEPES, 150mM NaCl, 1uM ZnCl2, 4mM TCEP, pH 8. Crystal: Crystal: crystal under UV m <b06fc576.png> <e091c7fd.png> <8ef9453e.png> When we collected the data at an in-house facility, it looked something like this: <b903961d.png> The minimum resolution spot is around 9Ang and maximum ~2.2Ang. I have not come across a protein diffraction like this, nor of a salt. When I ran the gel for the incubated protein (protein+ligand), there was no degradation. Although, I was sure there is some problem with this image I tried processing, which could not be, But indexing showed a unit cell of 11Ang, 11Ang, 46Ang in P3. which was quite expected for two of the axes but not the third. Can anyone please shed some light on this diffraction image? How can it happen? Thank you Regards Kavya ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
