The phosphorus absorption edge is about 5.8Å. I've had much better luck with 5-Br-U for anomalous phasing.
Molecular replacement with sub-structural fragments can also work: <https://scottlab.ucsc.edu/scottlab/reprints/2010_Scott_Methods.pdf> Yours sincerely, William G. Scott Professor, Department of Chemistry and Biochemistry and The Center for the Molecular Biology of RNA University of California at Santa Cruz Santa Cruz, California 95064 USA > On Sep 18, 2023, at 2:43 AM, Eleanor Dodson > <0000176a9d5ebad7-dmarc-requ...@jiscmail.ac.uk> wrote: > > I am afraid most scientists will use the most straightforward technique! > If SAD is available the PHOSPHATE backbone of DNA will provide sufficient > signal to allow SAD to work, and you get an unambiguous answer to whether it > is A-DNA or B or Z... > MR will usually work of course as well > Eleanor > > > On Mon, 18 Sept 2023 at 09:18, Natesh Ramanathan <nat...@iisertvm.ac.in> > wrote: > Dear Fu Xingke, > > Depends on what Nucleic Acid you are talking of. If it is RNA, you > can expect some sequence to tertiary structure correspondence so you might be > able to try more MR as compared to DNA. DNA may have double helical > architecture but less sequence to tertiary structure correspondence, and > hence DNA is less likely to have a 3D structure like RNA specific structure > for a sequence. > > SAD has become a straight forward method to avoid all these problems > to get ab-initio structure. So many go for it directly. > > Hope that helps. > Best wishes, > Natesh > > On Mon, 18 Sept 2023 at 13:36, fuxingke <fuxingke0...@163.com> wrote: > Dear Colleagues, > > Reacently, I find the structures of Nucleic acid are solved by > single-wavelength anomalous diffraction(SAD). So, why molecular replacement > (MR) not? > > Regards > > > > Best wishes, > > Fu Xingke > > Institute of Physics CAS > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > > > -- > ---------------------------------------------------------- > "Live Simply and do Serious Things .. " > - Dorothy Mary Crowfoot Hodgkin OM, FRS > > "In Science truth always wins" > - Max Ferdinand Perutz OM FRS > ---------------------------------------------------------- > Dr. Ramanathan Natesh > Associate Professor, > School of Biology and Center for High-Performance Computing (CHPC), > Founding and Current President of Cryo Electron Microscopy and 3 Dimensional > Image Processing Society of India (CEM3DIPSI), > Indian Institute of Science Education and Research Thiruvananthapuram > (IISER-TVM), > Maruthamala P.O., Vithura, > Thiruvananthapuram, 695551, Kerala, India > > nat...@iisertvm.ac.in > http://faculty.iisertvm.ac.in/natesh > > Researcher ID: http://www.researcherid.com/rid/C-4488-2008 > ORCID: http://orcid.org/0000-0002-1145-5962 > Vidwan-ID : 94134: http://iisertvm.irins.org/profile/94134 > PUBLONS: https://publons.com/author/1520837/ramanathan-natesh#profile > > Office Ph. 0091- 471-2778087 > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
