Re: [ccp4bb] Multi-lattice crystal data processing strategy for structure solution

[Rezaul Karim]

Hi Devbrat, Have you tried indexing using XDS multi-lattice processing?  It is a very useful indexing process for multi-lattice and/or 2nd lattice interference with the major one.  Best, Rezaul Karim, Ph.D. Structural Biology Scientist Nurix Therapeutics Inc. Sent from my iPhone On Nov 15, 2023, at 6:22 PM, Devbrat Kumar <devformed...@gmail.com> wrote:  Dear Prof John Thank you for your valuable time and insight. I will do as you have suggested. Warm Regards- Devbrat Kumar   On Wed, Nov 15, 2023 at 11:28 PM John Bacik <0000b45abf420e1f-dmarc-requ...@jiscmail.ac.uk> wrote: Hi Devbrat, here are a couple of other things to try: - When screening crystals use rastering to find regions of the sample where multiple lattices may be less problematic. If multiple lattices are observed, often regions on the crystal(s) close to the edge will not be as affected by twinning/multiple lattices. Also try using fine slicing if you are not already. - Try using AlphaFold to generate a model for the MR template. All the best, John On Wednesday, November 15, 2023 at 06:10:06 AM CST, Phil Evans <p...@mrc-lmb.cam.ac.uk> wrote: Is the space group really P2? P21 is MUCH more common Phil > On 14 Nov 2023, at 15:55, Devbrat Kumar <devformed...@gmail.com> wrote: > > sed data were integrated with the data reduction tool AIMLESS in the CCP4i2 suite. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1