Hi All, I just collected a lot of data for native crystals and Seleno-methionine derivative crystals. I have a couple of questions 1. How do I know which images to process and which ones to exclude which might be radiation damaged and not be good to further process? I have been told it is possible to cutoff the damaged images, how do I know where to cut it off? 2. I also collected MAD data at two wavelengths (Remote, and at the arithmetic mean of the peak and inflection) and three for some crystals (remote, peak, and inflection), How do I proceed about solving the structure with this data and how should I process them? Is there an advantage to 3 wavelengths over 2 for MAD? 3. For some crystals I collected multiple datasets from the same crystal. I have been told I can merge the datasets (images) into a single, larger dataset. How do I do that? Can I combine datasets from different crystals grown under the same conditions with the same material? Or only the same individual crystal? Is it advantageous to merge datasets like this? 4. When Elves indexes the crystal initially, it always thinks the space group is P4. When I finish data collection it thinks P21 21 2. Is this normal? Is elves just guessing wrong initially? Why would my space group be ambiguous? Our resolution is around 3 angstroms.
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