Apologies, my response was directly to the OP and not to the BB. I have forwarded the response to maintain the chain.
---------- Forwarded message --------- From: Nicholas Clark <ndcla...@buffalo.edu> Date: Fri, May 31, 2024 at 7:23 AM Subject: Re: [ccp4bb] Crystal optimization To: 白雪慧 <zb20193020...@cau.edu.cn> I had a protein that no matter what we did, only grew urchins very similar to what you’ve shown here. Even with extensive optimization, we were only ever able to grow urchins. Ultimately, the only way we were able to grow diffraction quality crystals and allowed us to solve the structure (with resolution ranging from 1.4- 2.2 angstroms, depending on ligand) was using MMS, as Tom suggested. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4157405/ Best, Nick Nicholas D. Clark, PhD (He/Him) University at Buffalo Department of Structural Biology Jacob's School of Medicine & Biomedical Sciences 955 Main Street, RM 5130 Buffalo, NY 14203 On Thu, May 30, 2024 at 10:34 PM 白雪慧 <zb20193020...@cau.edu.cn> wrote: > Thank you very much for your suggestions. I have a question. My crystal > grows microcrystals under multiple conditions, as shown in the figure. > After orthogonal optimization of the precipitant and pH, the crystal growth > is still very small and difficult to obtain diffraction. What is the method > to optimize and increase the crystal size in this situation? > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > <https://nam12.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2FWA-JISC.exe%3FSUBED1%3DCCP4BB%26A%3D1&data=05%7C02%7Cndclark2%40g-mail.buffalo.edu%7Cd15f0afd4af945855efb08dc811a166f%7C96464a8af8ed40b199e25f6b50a20250%7C0%7C0%7C638527196413692164%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=xWKfAAHjMInPToUjqIzNO99zljdso01mAd478t1qp%2Fk%3D&reserved=0> > -- Nicholas D. Clark, PhD (He/Him) University at Buffalo Department of Structural Biology Jacob's School of Medicine & Biomedical Sciences 955 Main Street, RM 5130 Buffalo, NY 14203 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
