Hi,
It seems to my eyes that you have multiple nucleation events happening
simultaneously. I would recommend you to try crystallization under 10 or 4 ºC
Best of luck
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestre em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São Carlos
phone: +55 16 99766-0021
"A sorte acompanha uma mente bem treinada"
________________________________________________
________________________________
De: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> em nome de 白雪慧
<zb20193020...@cau.edu.cn>
Enviado: Friday, May 31, 2024 3:32:52 AM
Para: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK>
Assunto: [ccp4bb] Crystal optimization
Thank you very much for your suggestions. I have a question. My crystal grows
microcrystals under multiple conditions, as shown in the figure. After
orthogonal optimization of the precipitant and pH, the crystal growth is still
very small and difficult to obtain diffraction. What is the method to optimize
and increase the crystal size in this situation?
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