Dear all,

As I somehow identify with the mention of "some ayatollahs” who perhaps 
underwent several rounds of meticulous refinement of demeanor, I fail to see 
any wrong to what Oliviero wrote. Oliviero, explains that a third party 
proclaimed that "unfortunately, the crystallographic programs became so 
automatic and easy to use nowadays, that even complete ignorant people can get 
something from them, and do not think if what they got makes sense or not.”. He 
is not offending anyone in particular or in general as ignorant. He is simply 
stating, that “ignorant people” unfortunately exist, and are responsible for 
atrocities in the PDB. I would venture to further proclaim that ignorant people 
exist in all walks of life and are responsible for worse atrocities than the 
occasionally unreasonable assignment of TLS groups. I am not claiming these 
people are ignorant by choice, and I am not claiming they are here reading this 
BB - if they were reading, they would not be as ignorant, or at least they 
would be actively trying to limit their ignorance.

Having previously limited my ignorance in X-ray crystallography largely due to 
reading this BB, and having my ignorance exposed to this BB, I fail to see 
anything wrong with good old-fashioned ignorance. And this BB has a large 
willingness to cure ignorance. But, lets face a fact: unfortunately, the 
crystallographic programs became so automatic and easy to use nowadays, that 
even complete ignorant people can get something from them, and do not think if 
what they got makes sense or not. There are such people out there, sorry to say 
that. Ignorance exists. The ignorant is not the same as the non-expert.  Anyone 
who writes here asking any question, is by definition not ignorant: by 
communicating here we all make a first crucial step, acknowledging the limit of 
our knowledge (which is the extend of our ignorance). And we ask to learn. 
These are in my view the non-experts that Gerlind is referring to, and are here 
to learn, and many try to help (I admit I am not helping much the last decade, 
mostly as I am hands-off lately). But, the non-expert crowd, is not the same as 
the ignorant crowd. They are different.

My best regards to all and specifically to my good and thoughtful and very 
gentle and kind friends Oliviero and Gerlind.

Apologies for my long and largely irrelevant rant -

Tassos


On 29 Oct 2024, at 17:08, Gerlind Sulzenbacher 
<[email protected]> wrote:

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Dear Italo,

It seems to me that the ccp4bb has always been a very gentle and empathic 
platform of exchange of ideas and offering a great deal of help to non-experts, 
"ignorants" as your friend calls them.

I remember that in the late 90thies there were some ayatollahs, which have 
become gentle civils by now, that used accusatory citations to underpin their 
claims. This period is over, or at least I hope so.

If ignorance exists, it's our fault of not being able to teach properly the 
young generation.

I understand your frustation and may be it deserves a letter in an Acta 
Journal, but please, please, refrain of citing the "ignorants" in this bb.

Wishing you a happy retirement and nice and fruitfuil projects, other than 
B-factors, for your future.

Gerlind



On 29/10/2024 16:38, Italo Carugo Oliviero wrote:

Ce mail provient de l'extérieur, restons vigilants
In a few months, I will retire, because my brain thinks it is still young but 
the rest of the body disagrees and thinks the brain is slightly senile. Of 
course, I will miss the protein structures very much, so unpredictable in their 
insolent beauty.

I have devoted several years of my life to the B-factors of their atoms.

Initially, almost 30 years ago, some colleagues mocked me, telling me that the 
B-side of proteins was nothing but dust hidden under the carpet, nothing but 
background noise. Some later changed their minds, and it is now generally 
agreed that the B-side of proteins deserves to be visited and admired.

However, I am concerned about more recent developments that are turning 
B-factors into real junk. TLS refinements as audacious as they are ridiculously 
unprofessional are producing disasters. For example, the 8q66 structure is very 
strange. It has been split into individual TLS mini-blocks, which clearly 
demonstrates that the authors are unaware of the TLS procedure and its proper 
application. A protein containing two structural domains was divided into six 
small TLS blocks, one of which belongs to both domains. Where are physics and 
chemistry?

How can this happen? Here is the answer from a friend and colleague: 
“unfortunately, the crystallographic programs became so automatic and easy to 
use nowadays, that even complete ignorant people can get something from them, 
and do not think if what they got makes sense or not.”


Are computational models preferable in the end?

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--
Gerlind Sulzenbacher
Architecture et Fonction des Macromolécules Biologiques
UMR7257 CNRS, Aix-Marseille Université
Case 932
163 Avenue de Luminy
13288 Marseille cedex 9
France
Tel +33 413 94 95 27
E-mail: 
[email protected]<mailto:[email protected]>


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