Note the initial empty <option>.  Also, note that you only need 1 set of 
enclosing <cfoutput> tags.

<cfset lookupOptionsArray = listtoarray("Blood,DNA,Other") />

<cfselect name="sampleType" size="1" multiple="no" style="width:200px" 
required="yes" message="Please select a Sample Type to continue.">
       <option value=""></option>
     <cfoutput>
     <cfloop index="i" from="1" to="#arraylen(lookupOptionsArray)#">
          <option
value="#lookupOptionsArray[i]#">#lookupOptionsArray[i]#</option>
     </cfloop>
     </cfoutput>
</cfselect>
 



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