Hi all,
I have experienced strange behavior of Align&mutate tool. I wanted to
correct few differences in sequence with it, and there is a place in the
sequence looking like this:
orginal sequence:
XYZDSUVW
new sequence:
XYZSPUVW
which ends after Align&mutate like this:
XYZDS-UVW
XYZ-SPUVW
and more over numbering of residues changes because of it. And that is
wrong.
Therefore, I suggest to incorporate an option "Treat sequence rigid" or
something like that, which would just found best match without any
"missing" residues. Yes, Mutate residue range did the trick, but it has
slightly different purpose, I think.
The second problem I have noticed, that it seems that with both
mutate-sequence tools (Align&mutate, Mutate residue range), it mutates
all the residues, including those which remains same. That cause
displacement of correctly placed residues.
Best regards,
Jan
--
Jan Stransky, PhD student
Institute of Biotechnology, AS CR
Laboratory of structure and function of biomolecules
Nad Safinou II 366
Vestec
Czech Republic
Tel.: +420226201570
