On 19/03/2021 15:27, M.S. Islam wrote:

I have done rigid body fitting of a couple of chains of "A form RNA" into an EM map using Chimera.

Why not do it in Coot? Or, put it another way: what does Chimera give you for rigid body fitting that Coot does not?

Now I intend to join the chains and renumber the residues according to RNA sequence on COOT.

OK.

Could anyone please share their experience of how to do this on COOT?

Merge Molecules, Change Chain IDs and Renumber Residues are Edit menu items.

Paul.

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