Dear Ray users and developers,
I'm using Ray in order to assemble an highly repetitive plant genome using Illumina and 454 reads. It is well known that the genome is composed of long stretches of microsatellites. Allowing ray to automatically set -minimumCoverage and -peakCoverage the estimated coverage and genome length is completely wrong. I'm interested in knowing how ray uses such parameters: it simply deletes all nodes in the k-mer graph that are composed by kmers that appear more than peakCoverage or it does some more sophisticated?

Moreover, how -repeatCoverage affects the assembler performances and the final result?

Thanks
Francesco

--

Francesco Vezzi, PhD Student
Tel: +39 3805151541
Department of Mathematics and Computer Science,
University of Udine, Udine, Italy
               &
Applied Genomics Institute (IGA), Udine, Italy
http://www.appliedgenomics.org


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