Hello, I have thousands of leaf discs from Helianthus annuus (see image at this link: http://web.me.com/scott_c1/Scott_Chamberlain/Other.html). I need to count the glandular trichomes (both the ones with and without glands on the end, i.e., the long hairs, and the white blobs) on the leaf discs. Ideally this would be an automated process using e.g., ImageJ. However, ImageJ is having a hard time discerning the trichomes from the rest of the leaf material. Here is my question:
Does anyone know how to stain just glandular trichomes, and not the remaining leaf material? With this, I could (more) easily count glandular trichomes in ImageJ. Sincerely, Scott Chamberlain Rice University, EEB Dept.
