Hi, I want to use the "restrict" program in EMBOSS package to do a restriction digest of a nucleotide sequence. When I ran restrict, it threw the following error;
EMBOSS An error in restrict.c at line 147: Cannot locate enzyme file. Run REBASEEXTRACT I believe this program should use a data file for restriction enzymes, Rebase. I noticed this is missing from our installation under ~/data/REBASE subdir. I just see a "dummyfile" there. I have a few questions on EMBOSS restriction analysis and will appreciate any ideas or thoughts on these. 1. What Rebase file we need to download to get "restrict" working? I tried but there are files with different formats. 2. Is there a maximum size limit of a nucleotide sequence that I can use? Can I use the whole Human genome or at least a full chromosome to digest with a particular restriction enzyme? 3. What program can give me the list of all possible fragments generated as well? Since I have not seen the output of "restrict", perhaps that is already doing that. Thanks for any suggestions. Perdeep Mehta Email Disclaimer: www.stjude.org/emaildisclaimer _______________________________________________ EMBOSS mailing list EMBOSS@lists.open-bio.org http://lists.open-bio.org/mailman/listinfo/emboss
