>>should I now rerun the bbregsiter and create a new dat file for the subfield
>> volume to orig.mgz image?

I did not entirely follow this thread. It sound like you have 3 volumes: 
orig.mgz, an EPI, and some highres hippo seg? How did you get the hires hippo 
seg? If it shares a scanner space with the orig.mgz, then run tkregister2 --mov 
hireshippo --s subject --fstarg --reg hip2orig.reg.dat --regheader

this will bring up the tkregister2 window and you can double check that it 
looks ok; save the reg. In the future you can just add --noedit and it will 
write the output reg without bringing up the interface.

but I might not understand what is going on ...
doug

On 04/02/2014 03:03 PM, Erik O'Hanlon wrote:
> Apologies.
>
> forwarded it on now
>
> Thanks a million
>
> Erik
>
> Dr. Erik O'Hanlon PhD MSc BScEng
> Post Doctoral Researcher
>
>
> RCSI Psychiatry
> Royal College of Surgeons in Ireland
> Beaumont Road, Beaumont, D9, Ireland
> T: 8093740
> E: erikohan...@rcsi.ie  W: www.rcsi.ie
>
> RCSI DEVELOPING HEALTHCARE LEADERS
> WHO MAKE A DIFFERENCE WORLDWIDE
> ________________________________________
> From: Bruce Fischl [fis...@nmr.mgh.harvard.edu]
> Sent: 02 April 2014 19:58
> To: Erik O'Hanlon
> Subject: Re: [Freesurfer] FW: registration difficulty
>
> Hi Erik
>
> can you cc the list so that others (like Doug!) can respond?
>
> thanks
> Bruce
> On Wed, 2 Apr
> 2014, Erik O'Hanlon wrote:
>
>> Hi Bruce,
>>
>> Thanks so much for your reply. As for the specifics of what I have tried, so
>> rry if it was unclear.
>>
>> I have performed the usual T1 based FS cortical and subcortical analysis and
>>   included the -hip_subfields so I also get all the subdivision volumes. I ha
>> ve also run a DWI analysis in ExploreDTI and now want to use the subfield vo
>> lumes as masks for the purpose of tractography. I created my diff-to-orig da
>> t file using bbregister. I then used this in the mri_vol2vol and aligned my
>> aseg.mgz to my nodif image. This worked really well and I then used fslmaths
>>   to extract out the hippocampus from the aseg2diff. This aligns nicely. I th
>> en tried to apply the same transformation to my subfield volumes but this di
>> dn't work. As you suggested, and just to clarify,
>>
>> should I now rerun the bbregsiter and create a new dat file for the subfield
>>   volume to orig.mgz image? Do I then use this in the mri_vol2vol and align m
>> y subfield volume to the orig.mgz and then run a second mri_vol2vol but use
>> the nodif2orig.dat calculated before and use the nodif image as the --mov fi
>> le and the registered subfield_2orig.nii  as the --targ and use the --inv --
>> nearest settings as I want end up with my subfield volumes in diffusion spac
>> e ?
>>
>> So sorry for the confusion, really shows my lack of experience with FS. Just
>>   getting completely mixed up at this stage.
>>
>> Again apologies for the lack of clarity and confusion but any pointers offer
>> ed would be fantastic.
>>
>> Thanks again
>>
>> best regards
>>
>> Erik
>>
>> __________________________________________________________________
>>
>> Hi Erik
>>
>> so you had a separate (presumably highres) acquisition within the
>> hippocampus? Have you registered that with bbregister? Your best bet is
>> probably doing so, then composing the two registrations (and inverting one,
>> depending on what direction you want to go).
>>
>> cheers
>> Bruce
>>
>>
>>
>> On Wed, 2 Apr 2014, Erik O'Hanlon wrote:
>>
>>>
>>> Dear FS experts,
>>>
>>> I am running my first FS analysis and I'm trying to register my hippocampa
>> l
>>> subdivision volumes to my diffusion space image but cannot get good
>>> alignment. I ran my diffusion preprocessing in ExploreDTI so don't have th
>> e
>>> register.dat file automatically so created this using the bbregister tool
>> and
>>> registered B0 image to my orig.mgz. I then used this dat to align my aseg
>> to
>>> my diffusion space image and it worked well. The issue is when I try to us
>> e
>>> it to transform my hippocampal subfield volumes to diffusion space. They j
>> ust
>>> don't line up. Is this due to the different FOV for the subfields? Can any
>> one
>>> advise how to get these volumes to transform correctly?
>>>
>>> Any help would be greatly appreciated.
>>>
>>> Thanks
>>>
>>> Erik
>> Dr. Erik O'Hanlon PhD MSc BScEng
>> Post Doctoral Researcher
>>
>> [IMAGE]
>>
>> RCSI Psychiatry
>> Royal College of Surgeons in Ireland
>> Beaumont Road, Beaumont, D9, Ireland
>> T: 8093740
>> E: erikohan...@rcsi.ie  W: www.rcsi.ie
>>
>> RCSI DEVELOPING HEALTHCARE LEADERS
>> WHO MAKE A DIFFERENCE WORLDWIDE
>>
>>
>>
>>
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-- 
Douglas N. Greve, Ph.D.
MGH-NMR Center
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