Hi Anatasia,

Thank you for your reply, I'm sorry for responding so later, I have been
trying a few different things in order to get tracula to work. I did what
you suggested and compiled the bvals and bvecs into one file, in the same
order as the images. This got me further, but the trac-all -prep step is
still not completing without error. The lastest problem I can't get past is
this:

"WARNING!!!! Multiple image files detected:
/users/marchaut/Documents/myprojects/R01/TESTS/TEST1/dmri/dwi.nii
/Users/marchaut/Documents/myprojects/R01/TESTS/TEST1/dmri.dwi.nii.gz

STOPPING PROGRAM
Darwin Marcs-Macbook-Pro.local 13.4.0 Darwin Kernel Version 13.4.0: Mon Jan
11 18:17:34 PST 2016, root:xnu-2422.115.15~1/RELEASE_X86_64 x86_64

trac-preproc exited with ERRORS at Fri Nov 11 14:46:42 EST 2016"


But since I'm combining the AP and PA images files, wouldn't it be
necessary for there to be multiple image files?

Marissa


On Wed, Sep 14, 2016 at 9:54 PM, Anastasia Yendiki <
ayend...@nmr.mgh.harvard.edu> wrote:

>
> Hi Marissa - You have to specify as many b-values and gradient vectors as
> there are volumes in your DWI data file. This means that, if you combine
> the AP and PA volumes, you also have to concatenate the bvals from the two
> into one file, and do the same with the bvecs. Make sure that the order in
> which the AP and PA volumes are combined is the same as the order in which
> the corresponding bvals and bvecs are combined.
>
> Hope this helps,
>
> a.y
>
>
> On Wed, 14 Sep 2016, Marissa Pifer wrote:
>
> Hello freesurfer experts,
>> I am trying to run tracula on subjects that have separate AP/PA direction
>> DTI files. We combined the data
>> into one using topup, and would now like to run tracula on the subject.
>> The problem is, the combined file
>> doesn't have a bvals/bvecs file. When I try to use the original bval/bvec
>> files, I get the error that the
>> bvals and bvecs don't match up. I have tried combining the files into
>> one, and then running a dicom to
>> nifty conversion to get a combined bvals/bvecs output, but that results
>> again in two separate bvals/bvecs
>> files as well. I then tried just have two specified bvals and bvecs. This
>> did not give us an error
>> message, but trac-prep took only a few seconds and did not complete the
>> steps it was supposed to to create
>> the directories for trac-bedp to complete. I have attached our
>> dmrirc.example file and the log for the
>> trac-prep step for your reference.
>>
>> The questions then are; is there a way to get one bvals/bvecs file that
>> has the correct parameters for the
>> AP and PA combined file. Or, if not, is there a way to run tracula with
>> two sets of bvals and bvecs (one
>> for the AP and one for the PA direction)?
>>
>> In case you need this information, the process is being run with
>> freesurfer 5.3, on a macbook pro OS X
>> version 10.9.5.
>>
>> Thank you in advance,
>>
>> Marissa
>>
>>
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