Hello all, FAO: Administrators of local Galaxy instances using the NCBI BLAST+ wrappers.
Over on the galaxy_blast repository I have been updating the NCBI BLAST+ wrappers (including unit tests) to work with the current release, NCBI BLAST+ 2.2.28 (aka BLAST 2.2.28+): https://github.com/peterjc/galaxy_blast The initial set of changes is now on the Test Tool Shed, http://testtoolshed.g2.bx.psu.edu/view/peterjc/ncbi_blast_plus This includes a workaround for a known regression in the makeblastdb tool dealing with duplicated identifiers: https://github.com/peterjc/galaxy_blast/commit/349e31c6cec4429c5523fde5975e28e399e0dfb1 In terms of end-user features, the big improvement in the BLAST+ 2.2.28 release was the ability to get the BLAST match descriptions in the tabular output, and other fields: http://blastedbio.blogspot.co.uk/2012/05/blast-tabular-missing-descriptions.html staxids means Subject Taxonomy ID(s), separated by a ';' sscinames means Subject Scientific Name(s), separated by a ';' scomnames means Subject Common Name(s), separated by a ';' sblastnames means Subject Blast Name(s), separated by a ';' (in alphabetical order) sskingdoms means Subject Super Kingdom(s), separated by a ';' (in alphabetical order) stitle means Subject Title salltitles means All Subject Title(s), separated by a '<>' sstrand means Subject Strand qcovs means Query Coverage Per Subject qcovhsp means Query Coverage Per HSP On this branch I am including the new salltitles field as the 25th column in the extended BLAST tabular output offered within the Galaxy interface: https://github.com/peterjc/galaxy_blast/tree/c25 However, I'm not so sure about the taxonomy fields. Since (thus far) they are not available via the XML, I am leaning to introducing a third tabular mode, e.g. * Standard 12 columns (can convert from XML) * Extended 25 columns (can convert from XML) * Extended also with taxonomy (cannot currently convert from XML) Instead, we could offer a pick-you-own columns route (in all the primary BLAST tools, handled via macros)?: * Standard 12 columns (can convert from XML) * Extended 25 columns (can convert from XML) * Pick your own columns from the full NCBI list (depending on columns, can convert from XML) This is inspired by JJ's changes to the BLAST XML to tabular conversion tool for Galaxy-P, https://github.com/jmchilton/galaxy_blast/commit/d79afc03522768323494818a40aac10513a6fa09 I would be much keener on the pick-you-own columns option if it was possible for the tool to record arbitrary column names for a tabular file in Galaxy's metadata (I can't find a trello card, but I'm sure I've asked about this before). Any thoughts or comments? eg Hurry up and just release this branch adding the hit descriptions as column 25 - we want that now ;) [*] Regards, Peter [*] For our local instance, the taxonomy stuff will be useful, but right now I would prioritise the description, which we currently get via the BLAST XML using this tool: https://github.com/peterjc/galaxy_blast/tree/master/tools/blastxml_to_top_descr ___________________________________________________________ Please keep all replies on the list by using "reply all" in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/
