Hi Isabelle,

Not sure about specifying the absolute path for the tool dependency dir, but I 
always use it. Also, my env.sh neither has a #!/bin/bash shebang line, nor 
quote marks around the tool path.
If you are in the hhsuite/default/ dir and source env.sh, can you then run 
hhblits yourself (as galaxy user, or whoever owns the tools)?

cheers,
— 
Jorrit Boekel
Proteomics systems developer
BILS / Lehtiö lab
Scilifelab Stockholm, Sweden



On 31 May 2014, at 01:53, Isabelle Phan <isabelle.p...@seattlebiomed.org> wrote:

> Hello,
> 
> I'm following "Managed Tool Dependencies" to the letter and still can't
> get Galaxy to find the executable. Is this page
> https://wiki.galaxyproject.org/Admin/Config/ToolDependencies up to date?
> If yes, what am I doing wrong?
> 
> Any help greatly appreciated!
> 
> 
> # universe_wsgi.ini
> # I also tried the absolute path, it made no difference
> tool_dependency_dir = tool_dependencies
> 
> # tool_dependencies is set like instructed:
> 
> $ ls tool_dependencies/hhsuite/
> 2.0.16/  default@
> $ ls tool_dependencies/hhsuite/2.0.16/bin
> 
> ffindex_build* ffindex_get*   hhalign*       hhblits*       hhconsensus*
> hhfilter*      hhmake*        hhsearch*
> 
> 
> My tool:
> <tool id="hhpred_hhblits_01" name="Create MSA from single fasta for
> hhsearch" hidden="false" version="1.0.0" force_history_refresh="true">
>       <requirement type="package">hhsuite</requirement>
>       <description>Searches single fasta iteratively against hmm db to build a
> MSA</description>
>       <command>hhblits -i $input_file -d $database -oa3m $outfile</command>
> etc...
> 
> 
> Galaxy throws Error: hhblits not found.
> 
> 
> I've tried setting the PATH in the tool_dependencies/hhsuite/2.0.16/env.sh:
> #!/bin/bash
> # configure PATH to hhsuite binaries
> PATH="/opt/galaxy-dist/tool_dependencies/hhsuite/default/bin:$PATH"
> export PATH
> 
> 
> 
> still getting hhblits not found :-(
> What am I doing wrong?
> 
> Isabelle
> 
> 
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