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Hello,

Chromosome names will need to be modified once the file is imported, as explained in #5 of the FAQ:
http://usegalaxy.org/u/jeremy/p/transcriptome-analysis-faq

Hopefully this helps,

Best,
Jen
Galaxy team

On 9/15/11 8:22 PM, shamsher jagat wrote:
I have related question If I have to use Ensembl mouse GTF file
(Mus_musculus.NCBIM37.64) Do I have to download and reformat it or
Galaxy can take it from the source directly?

Thanks

On Sun, Aug 28, 2011 at 8:50 AM, Peng, Tao <tp...@fhcrc.org
<mailto:tp...@fhcrc.org>> wrote:

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    __

    Hi how can I specify a GTF gene annotation file when running tophat
    to guide the alignment to human genome? What is the best way to
    visualize the tophat results in the context of annotated human
    genome, i.e. RefSeq?

    Thanks,

    tao


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___________________________________________________________
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