Good Morning Meri:

You can read the sequence of events in the alignment procedure
in the text file:

http://hgwdev.cse.ucsc.edu/~kent/src/unzipped/hg/makeDb/doc/sacCer2.txt

Look for the section starting:
############################################################################
# DOING MULTIPLE ALIGNMENT WITH OTHER YEAST  (DONE - 2009-02-10 - Hiram)

It looks like lastz was used without any arguments and the version was:
   lastz-- Local Alignment Search Tool, blastZ-like
   (version 1.00.05 built 20080802)

I have packaged the pair wise alignments in lav and psl format
in the tar gzipped file:
http://hgwdev.cse.ucsc.edu/~hiram/sacCer2/sacCer2.pairwise.tgz

--Hiram

meritxell oliva pavia wrote:
> Dear UCSCers,
> 
> I'm interested in comparing the performance of various multiple genome 
> alignment methods in a yeast 7-way genome alignment. This whole-genome 
> alignment is precomputed and available in UCSC web
> http://hgdownload.cse.ucsc.edu/goldenPath/sacCer2/multiz7way/
> 
> As far as I know, this alignment was created with the Penn State multiz 
> alignment tool applied to pairwise alignments which are produced by a 
> pipeline consisting of the Penn State blastz aligner, followed by the 
> UCSC chain/net process. I would like to know if blastz pairwise local 
> alignments used as input for the 7-way alignment are available somewhere 
> through the UCSC web.  Could you provide me the BLASTZ parameters you 
> used for the alignments, as well as the BLASTZ version? (BLASTZ was 
> replaced by LASTZ, I don't know up to which point the results for each 
> method are similar)
> 
> Thanks in advance,
> 
> Meri

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