Hi,

 

I'd like to report a strange bug: when making custom track/bed file of
the knownGene exons using Table tool for the whole genome I get a number
of exons that are not in actual knownGene track.

For example more than 100 extra exons are listed for region
chr15:22787248-22813789 (hg18). That region has only 3 ucsc genes
overlapping:

 

chr15    22751162          22795318          uc001ywz.1       0
+          22774020          22776299          0          6
140,96,139,126,138,109,            0,7191,22855,23270,25024,44047,

chr15    22751162          22795318          uc001yxa.1       0
+          22751162          22751162          0          7
140,96,151,107,138,663,109,      0,7191,13009,24613,25024,26852,44047,

chr15    22778014          22817591          uc001yxc.2       0
+          22778014          22778014          0          9
663,121,109,130,356,164,122,42,762,0,16591,17195,18470,19583,20950,37252
,38053,38815,

 

and  correspondingly 22 exons. This could be seen when making exon track
for the region only. However when creating such track for the whole
genome (hg18)  the same region on chr15 has 139 exons, listing 119 extra
exons, Below are just a few of those (let me know if you want me to post
whole list):

 

chr15    22758353          22758449
uc001ywp.1_exon_4_0_chr15_22758354_f          0          +

chr15    22764171          22764322
uc001ywy.1_exon_2_0_chr15_22764172_f          0          +

chr15    22765034          22765069
uc001ywu.1_exon_3_0_chr15_22765035_f          0          +

chr15    22770527          22770696
uc001ywx.1_exon_3_0_chr15_22770528_f          0          +

chr15    22770550          22770696
uc001ywp.1_exon_6_0_chr15_22770551_f          0          +

chr15    22770881          22771197
uc001ywv.1_exon_5_0_chr15_22770882_f          0          +

chr15    22771597          22771749
uc001ywy.1_exon_4_0_chr15_22771598_f          0          +

chr15    22772544          22772656
uc001ywp.1_exon_8_0_chr15_22772545_f          0          +

chr15    22772544          22772656
uc001ywy.1_exon_5_0_chr15_22772545_f          0          +

chr15    22773116          22773269
uc001ywp.1_exon_9_0_chr15_22773117_f          0          +

chr15    22774017          22774156
uc001ywy.1_exon_7_0_chr15_22774018_f          0          +

chr15    22774432          22774558
uc001ywp.1_exon_11_0_chr15_22774433_f        0          +

chr15    22774646          22774822
uc001ywp.1_exon_12_0_chr15_22774647_f        0          +

chr15    22778233          22780030
uc001yxb.2_exon_0_0_chr15_22778234_f           0          +

chr15    22781099          22784472
uc001yxd.2_exon_0_0_chr15_22781100_f           0          +

 

Could you please check this? I haven't noticed similar problem for any
other gene prediction tracks, only for known genes.

 

Thanks!

 

Irina Khrebtukova

Illumina, Hayward CA

 

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