Hi Galt, thanks for the answer. Via command line this is the input : gfClient -t=dna -q=rna $HOST $PORT hg19.2bit $infile $outfile
Via Web these are the selected options: Genome:Human; Assembly:GRCh37/hg19; QueryType:DNA; Sort Output:Query Score; Output Type:PSL; Thanks again. Ps. Why the output of gfClient-Server is labeled psLayout version 3 instead of psLayout version 4 of hgBlat ? On May 15, 2010, at 1:32 AM, Galt Barber wrote: > > Hi, Mauro! > > In order to help, we need some more information: > > What commandline was used to query with gfClient? > > What commandline was used to start gfServer? > > -Galt > > Mauro Donadello wrote: >> Hi, i've this sequence : >>> hsa-mir-17 MI0000071 >> GUCAGAAUAAUGUCAAAGUGCUUACAGUGCAGGUAGUGAUAUGUGCAUCUACUGCAGUGAAGGCACUUGUAGCAUUAUGGUGAC >> if I use hgBlat(webBlat) of our mirror , I obtain these results : >> Genome:Human; Assembly:GRCh37/hg19 >> ACTIONS QUERY SCORE START END QSIZE IDENTITY CHRO STRAND >> START END SPAN >> --------------------------------------------------------------------------------------------------- >> browser details hsa-mir-17 84 1 84 84 100.0% 13 + >> 92002859 92002942 84 >> browser details hsa-mir-17 33 10 56 84 89.2% X - >> 133304255 133304300 46 >> browser details hsa-mir-17 22 43 65 84 100.0% 6_qbl_hap6 >> + 2248519 2248544 26 >> browser details hsa-mir-17 22 43 65 84 100.0% 6_mann_hap4 >> + 2303515 2303540 26 >> but when I use gfClient there are no results. >> gfClient and gfServer are compiled from 2.30 gb ucsc source code. Any idea ? >> Thanks a lot. _______________________________________________ >> Genome maillist - [email protected] >> https://lists.soe.ucsc.edu/mailman/listinfo/genome _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
