Dear Kevin,

The differences that you are seeing are most likely due to the different 
versions of RepeatMasker that are being used. In general, successive releases 
of RepeatMasker and the library that it uses (RepBase Update from 
http://www.girinst.org) find more and more repeats in genomic sequence.

I hope that this helps. If you have have further questions, please don't 
hesitate in contacting the mailing list again.

Vanessa Kirkup Swing
UCSC Genome Bioinformatics Group


----- Original Message -----
From: "Kevin Gunderson" <[email protected]>
To: [email protected]
Sent: Friday, August 27, 2010 3:45:24 PM GMT -08:00 US/Canada Pacific
Subject: [Genome] Non-conservation of repeat masked sequence between hg18 and 
hg19 builds

To Whom It May Concern:

 

            Why isn't repeat masked sequence conserved between hg18 and
hg19 builds?  Are different repeat masker algorithms used?  As an
example I have fetched some sequence around SNP rs10493230.

 

Masked sequence (lower case) not conserved between builds

 

rs10493230-Hg18

TCTGGGTCCTTGGAATAGTGTGCTCCTTCCATAGCTGAAATCTTTGGTCCTAGACATAGTCCCTGTACTCTG
CAACCACTGATACCATGTTGCTTCCAGTGACCTGGGCTCGGCACCTCTGCATTCCCTCCCTGGGATATCCTA
ATGTCTCCCTGAACAGGCACTAGGGAAACAGAAATATCACTCAGACCTATTATTCCAGACATGTCTATACAC
ACATATATTATGCTCTCCTAAGGAACAAGGTGCCAAGCCAAATGCAGAACATTCTCTAGAGCCTTGTAGTTA
CTCTTCCAAGCAATGCCTAGAGAAGTAGTCTCCAGCTTTCTGTGTTGTTAGGTCCCCTAATACCTGTATGGA
TATCTCCATTATTAGTTTCTCTACCCCTTGTCAAGATATGGCTATGTGGCAGGAGGTAGAATGAGAGATTAG
TAAGAATCCTGCCATCACATTTCCTCATCTACTTCTTCCTCACTGGAAGCAAATTCCTCTATATCGAATTCT
TTCTCCATTAAGACTCACACCTCggccaggcgcggtggcttacgcctgtaatcccaacacttgggaggccaa
ggtgggcagatcacaaggtcaggagttcgagaccagcctggccaacatgttgaaacctcgtctctactaaaa
atacaaaaattagctggatgtggtggtgggtggctgtaatgccagctactcgggaggctg

 

rs10493230-Hg19

tctgggtccttggaatagtgtgctccttccatagctgaaatctttggtcctagacatagtccctgtactctg
caaccactgataccatgttgcttccagtgacctgggctcggcacctctgcattccctccctgggatatccta
atgtctccctgaacaggcactagggaaacagaaatatcactcagacctattattccagacatgtctatacac
acatatattatgctctcctaaggaacaaggtgccaagccaaatgcagaacattctctagagccttgtagtta
ctcttccaagcaatgcctagagaagtagtctccagctttctgtgttgttaggtcccctaatacctgtatgga
tatctccattattagtttctctaccccttgtcaagatatggctatgtggcaggaggtagaatgagagattag
taagaatcctgccatcacatttcctcatctacttcttcctcACTGGAAGCAAATTCCTCTATATCGAATTCT
TTCTCCATTAAGACTCACACCTCggccaggcgcggtggcttacgcctgtaatcccaacacttgggaggccaa
ggtgggcagatcacaaggtcaggagttcgagaccagcctggccaacatgttgaaacctcgtctctactaaaa
atacaaaaattagctggatgtggtggtgggtggctgtaatgccagctactcgggaggctg

 

Thanks,


Kevin

 

Kevin Gunderson, Ph.D.

Senior Director, Advanced Research

Illumina, Inc. (www.illumina.com <http://www.illumina.com> )

9885 Towne Centre Dr

San Diego, CA 92121

Voice: (858) 202-4591

Email: [email protected]

 

 

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