Hello Julinor, Following your instructions for the query, I was able to find the match by clicking on the "Flip Reverse Primer" checkbox.
Try this and let us know if it does not address your problem, Jennifer Jackson UCSC Genome Bioinformatics Group Bacani, Julinor wrote: > Re: human March 2006, in silico PCR No matches result. > > I am looking at CDH1/E-cadherin gene to identify known SNPs. I was very > successfully 2 days ago upon checking my exons 1 and 2 primer sets. Today, > not changing anything and redoing what I had done to check exons 1 and 2 > again, instead of getting the desired sequences and SNPs, I got this: > > No matches to tacggggggcggtgcct caagctccgcgccccag in Human May 2006 > > I even tried earlier versions such as Human July 2003 > > I am not sure why all of a sudden this is happening. Does it have anything > to do with the new release Feb 12, 2009? > > Hoping for help soon, > > Sincerely, > > Julinor > _______________________________________________ > Genome maillist - [email protected] > http://www.soe.ucsc.edu/mailman/listinfo/genome > _______________________________________________ Genome maillist - [email protected] http://www.soe.ucsc.edu/mailman/listinfo/genome
