Hi Yuval, We are now investigating your issue with the BlatBot.pl program by testing it locally. Your IP is not currently blocked (the blocks decay after a certain amount of time).
Meanwhile, we have a few questions: 1) Do you get any results back? Or does the entire run fail? 2) When did you last download/update your version of BlatBot.pl? Downloading the most current version again is recommended as a first step to troubleshoot, since this will ensure we are both using the same program while we test. 3) Have you made any changes to the program? Thank you for the information and we will keep you updated about our testing progress, Jennifer Jackson UCSC Genome Bioinformatics Group Yuval Itan wrote: > Hi Jennifer, > > Thanks for attempting to help, truly appreciated. I tried again now to > use BlatBot.pl and I received a "connection timed out" error, and then > trying again I received an "internal server error". I'm a bit > surprised about the over-use from my side, since I am only trying to > Blat a few hundred of sequences (each not very long) using your script > that should limit it to a maximum of 25 per batch. Hopefully there is > a way to figure that out, if not then I'll probably just need to > manually do it via your website. > > Thank you, > > Yuval Yuval Itan wrote: > Hello, > > I am trying to use BlatBot.pl for 305 human peptide sequences that I > have. Every time I do it the run is terminated about halfway through > with the message - "did not receive an is_success, dying, content > is:500 Internal Server Error at BlatBot.pl line 112, <FH> line 977". I > was wondering if you have any suggestion of how to solve that, since a > few months ago I used BlatBot.pl and it was just fine. > > Thank you, > > Yuval > > _______________________________________________ > Genome maillist - [email protected] > http://www.soe.ucsc.edu/mailman/listinfo/genome > _______________________________________________ Genome maillist - [email protected] http://www.soe.ucsc.edu/mailman/listinfo/genome
