On 3/9/11 1:29 PM, Vanessa Kirkup Swing wrote:
> Hi Duke,
>
> Here is an easier way to get the 3' UTR sequences with refSeq ID's.
>
> In the table browser, please select the following:
>
> group: Genes and Gene Prediction track
> track: RefSeq Genes
> table: refGene
> region: genome
> output format: sequence
>
> Click "get output"
>
> Select: "genomic"
> Click "submit"
>
> Select: "3'UTRs"
> Click "get sequence"

Thanks so much Vanessa. It works perfectly!

D.

> Hope this helps! Please contact the mailing list if you have further 
> questions.
>
> Vanessa Kirkup Swing
> UCSC Genome Bioinformatics Group
>
>
> ----- Original Message -----
> From: "Duke"<[email protected]>
> To: [email protected]
> Sent: Tuesday, March 8, 2011 2:33:26 PM
> Subject: [Genome] Extract 3' UTR sequence with refSeq
>
> Hi,
>
> Following this
> (https://lists.soe.ucsc.edu/pipermail/genome/2005-October/008882.html)
> instruction, I was able to extract the 3' UTR sequence from Table
> Browser, but the fasta headers are gene ID and other infos (for example:
> ">mm9_refFlat_Xkr4 range=chr1:3204563-3206102 5'pad=0 3'pad=0 strand=-
> repeatMasking=none").
>
> Please let me know a proper way of getting 3' UTR but with refSeq IDs
> instead of gene IDs. Converting from gene ID to refSeq in this case
> seems not to be a solution since there are lots of identical gene IDs
> (and range etc...). One example would be: ">mm9_refFlat_Tcea1
> range=chr1:4886446-4887990 5'pad=0 3'pad=0 strand=+ repeatMasking=none"
> (there are three such identifications).
>
> Thank you in advance,
>
> D.
> _______________________________________________
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