Dear Sir/Madam,

I have 2 questions for liftOver tool from UCSC Genome browser. I 
liftover from hg19 to 4 primate genomes: panTro3, ponAbe2, rheMac2 and 
calJac3. My input is 0-based start BED file. My questions are,

1) For panTro3, rheMac2 and calJac3, the output varies among different 
chromosomes. For instance, hg19 (chr1    14906    14907 A) can be 
successfully converted to panTro3 (chr15 99530063 99530064 A ), while 
hg19 (chr12   78301058    78301059 A) was converted to panTro3 (chr12 
77844433 77844434 A ) and the corrected one should be panTro3 (chr12 
77844434 77844435 A )

2) For ponAbe2, it seems the 0-based BED input will output -1 based BED 
file, so I use 1-based start BED file for ponAbe2. Is there any 
different between hg19-ponAbe2 chain file and other chain files?

Thanks in advance for your great help!

Qihui
----------------------------
Qihui Zhu, Ph.D.
Molecular Genetic Research Unit
Brigham&Women's Hospital | Harvard Medical School
221 Longwood Avenue, EBRC-404
Boston, MA 02115


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