Hello Qihui,

Thank you for your questions. The ponAbe2 chains were built in the same 
manner as the others and all follow our zero-based start and one-based 
end convention. For more information on this please see our FAQ:

http://genome.ucsc.edu/FAQ/FAQtracks.html#tracks1

And this wiki on coordinate transforms:

http://genomewiki.ucsc.edu/index.php/Coordinate_Transforms

Best regards,

Pauline Fujita,
UCSC Genome Bioinformatics Group
http://genome.ucsc.edu

On 09/14/11 09:14, Qihui Zhu wrote:
> Dear Sir/Madam,
> 
> I have 2 questions for liftOver tool from UCSC Genome browser. I 
> liftover from hg19 to 4 primate genomes: panTro3, ponAbe2, rheMac2 and 
> calJac3. My input is 0-based start BED file. My questions are,
> 
> 1) For panTro3, rheMac2 and calJac3, the output varies among different 
> chromosomes. For instance, hg19 (chr1    14906    14907 A) can be 
> successfully converted to panTro3 (chr15 99530063 99530064 A ), while 
> hg19 (chr12   78301058    78301059 A) was converted to panTro3 (chr12 
> 77844433 77844434 A ) and the corrected one should be panTro3 (chr12 
> 77844434 77844435 A )
> 
> 2) For ponAbe2, it seems the 0-based BED input will output -1 based BED 
> file, so I use 1-based start BED file for ponAbe2. Is there any 
> different between hg19-ponAbe2 chain file and other chain files?
> 
> Thanks in advance for your great help!
> 
> Qihui
> ----------------------------
> Qihui Zhu, Ph.D.
> Molecular Genetic Research Unit
> Brigham&Women's Hospital | Harvard Medical School
> 221 Longwood Avenue, EBRC-404
> Boston, MA 02115
> 
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