Hi, when using the human reference hg19 gene model, there are like this (in GTF output format from hgTable Genes and Gene Prediction group, RefSeq Genes track):
chr10 hg19_refGene start_codon 135480472 135480474 0.000000 + . gene_id "NM_012147"; transcript_id "NM_012147"; chr10 hg19_refGene CDS 135480472 135481677 0.000000 + 0 gene_id "NM_012147"; transcript_id "NM_012147"; chr10 hg19_refGene exon 135480432 135481677 0.000000 + . gene_id "NM_012147"; transcript_id "NM_012147"; chr10 hg19_refGene CDS 135484982 135485230 0.000000 + 0 gene_id "NM_012147"; transcript_id "NM_012147"; chr10 hg19_refGene stop_codon 135485231 135485233 0.000000 + . gene_id "NM_012147"; transcript_id "NM_012147"; chr10 hg19_refGene exon 135484982 135485275 0.000000 + . gene_id "NM_012147"; transcript_id "NM_012147"; where the hg19 model has an exon that does not exon exist in the RefSeq accession (or any historical version of the RefSeq accession). How/why does the alignment introduce an intron in this case? Does it ensure there are plausible flanking splice junctions before inserting an intron to a RefSeq sequence that lacks it but it maps to? Dan _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
