Hello UCSC Genome Browser Staff - We are experiencing difficulties with a custom track line for a .bam and indexed .bam file constructed from the training set described in the recent TopHat publication (RNA-seq with Drosophila).
We have the sorted and indexed files available at: http://usda02.vetmed.wsu.edu/public_bam/ However when we type the following into the custom track page: track type=bam name="My Bam" bigDataUrl=http://usda02.vetmed.wsu.edu/public_bam/accepted_hits.sorted.bam we get the following error: Can't access My Bam's bigDataUrl http://usda02.vetmed.wsu.edu/public_bam/accepted_hits.sorted.bam and/or the associated index file http://usda02.vetmed.wsu.edu/public_bam/accepted_hits.sorted.bam.bai: Error in TCP non-blocking connect() 111 - Connection refused bamFileExists: failed to read index corresponding to http://usda02.vetmed.wsu.edu/public_bam/accepted_hits.sorted.bam Is there something obvious that we're missing? Do we need to open a port (different than port 80)? Regards, David Herndon Molecular Biologist USDA-ARS Pullman, WA 99164-6630 509-335-6004 _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
