Thanks Yeti,
I'm trying, I'm not quite there yet! >From the file I can get the parameters below. I'm not sure how they correlate whith what Gwyddion asks for: What shall I choose in: binary data? What is the start at offset, how many bits for sample size, after each sample/row skip. As you can see my scan is 1umx1um with 512 lines are these the physical dimensions and resolution respectively? In this particular file, shall I use 15 nm as Z-scale? Thanks, FILE LIST Version v5.12r146606232 Start context OL2BIG Data length 40960 Engage X Pos -19783.4 um Engage Y Pos -42151.3 um CIAO IMAGE LIST Data offset 40960 Data length 524288 Bytes per pixel 2 Start context OL Data Type AFM Image Data Do zoffder 0 Note 1:20 dil Offset -96.107 nm Frame direction Up Stage X 0.00 µm Stage Y 0.00 µm Stage type N/A Profile length 0.00 µm Profile speed [um/s] 0.00 Capture start line 0 Number of samples 512 Number of lines 512 Aspect Ratio 1.00 Scan Size 1.00 µm Scan Line Main Line Direction Retrace Highpass Filter Off Lowpass Filter Off Realtime Plane Fit Line Offline Plane Fit Full Valid data start X 0 Valid data start Y 0 Valid data len X 512 Valid data len Y 512 Tip x width correction factor 1.00 nm Tip y width correction factor 1.00 nm Tip x width correction factor sigma 1.00nm Tip y width correction factor sigma 1.00nm Image Data Height Data Scale 10.00 nm Z scale 15.08 nm Data Center 0 nm -- Bibiana Onoa Bustamante Lab B308 AFM room Stanley Hall Berkeley,CA 94720-3220 Tel. +1(510) 666-2762
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