Hi

The HCP course tractography practical would be a good way to start (see page 
386 onwards):

https://wustl.app.box.com/s/wna2cu94pqgt8zskg687mj8zlmfj1pq7

Briefly, if you have a number of surfaces (need to convert them to ASCII using 
surf2surf) and subcortical volumes you can merge them into text files and use 
those as seeds. This will get you a dense connectome which you can then 
parcellate using any parcellation scheme you would like.

Best wishes
Stam


On 30 Oct 2018, at 23:35, Glasser, Matthew 
<glass...@wustl.edu<mailto:glass...@wustl.edu>> wrote:

Also use the label ones to make GIFTI label files.

Matt.

From: Leonardo Tozzi <lto...@stanford.edu<mailto:lto...@stanford.edu>>
Date: Tuesday, October 30, 2018 at 6:34 PM
To: Matt Glasser <glass...@wustl.edu<mailto:glass...@wustl.edu>>
Subject: Re: [HCP-Users] Diffusion connectivity matrix with cortical and 
subcortical parcellation

Dear Matthew,

Thank you for the very quick response.
So for clarification, you would use the following command:

wb_command -cifti-separate merged.dlabel.nii COLUMN -volume-all subcort.nii.gz

To obtain the subcortical volumes.

What about the cortical ones? Is the file 
Q1-Q6_RelatedValidation210.CorticalAreas_dil_Final_Final_Areas_Group_Colors.32k_fs_LR.dlabel.nii
 already usable as input to FSL? I thought I would need a .gii file. Or do I 
need to use the option in wb_command -cifti-separate:



[-label] - repeatable - separate a surface model into a surface label file.

In this case, what would be the argument for “<structure> - the structure to 
output” ?

Thank you very much,




Leonardo Tozzi, MD, PhD
Williams PanLab | Postdoctoral Fellow
Stanford University | 401 Quarry Rd
lto...@stanford.edu<mailto:lto...@stanford.edu> | (650) 5615738


From: "Glasser, Matthew" <glass...@wustl.edu<mailto:glass...@wustl.edu>>
Date: Tuesday, October 30, 2018 at 4:22 PM
To: Leonardo Tozzi <lto...@stanford.edu<mailto:lto...@stanford.edu>>, 
"hcp-users@humanconnectome.org<mailto:hcp-users@humanconnectome.org>" 
<hcp-users@humanconnectome.org<mailto:hcp-users@humanconnectome.org>>
Subject: Re: [HCP-Users] Diffusion connectivity matrix with cortical and 
subcortical parcellation

I would separate the CIFTI file into GIFTI and NIFTI components with wb_command 
-cifti-separate and follow the instructions for surface tracking on FSL’s 
website.

Matt.

From: 
<hcp-users-boun...@humanconnectome.org<mailto:hcp-users-boun...@humanconnectome.org>>
 on behalf of Leonardo Tozzi <lto...@stanford.edu<mailto:lto...@stanford.edu>>
Date: Tuesday, October 30, 2018 at 6:19 PM
To: "hcp-users@humanconnectome.org<mailto:hcp-users@humanconnectome.org>" 
<hcp-users@humanconnectome.org<mailto:hcp-users@humanconnectome.org>>
Subject: [HCP-Users] Diffusion connectivity matrix with cortical and 
subcortical parcellation

To Whom it may concern,


I have created a dlabel file by adding to the Glasser Parcellation from BALSA 
the subcortical regions of the Freesurfer atlas.

Now my goal would be to run a tractography analysis and obtain structural 
connectivity matrices using this cortical+subcortical parcellation. Could you 
suggest the simplest way to go from my dlabel file to something that is 
compatible with diffusion software? I know that probtrackx accepts GIFTIs as 
input, but I was wondering about how to extend this to subcortical areas. Or 
rather would the simplest solution be to convert each subject’s parcellation 
into a series of NIFTI files in native/standard space and input these as ROIs 
into probtrackx? In this case, could you recommend the correct series of 
workbench commands? I am also open to other software recommendations besides 
the FSL suite.
Thank you very much,



Leonardo Tozzi, MD, PhD
Williams PanLab | Postdoctoral Fellow
Stanford University | 401 Quarry Rd
lto...@stanford.edu<mailto:lto...@stanford.edu> | (650) 5615738

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