Dear histonet users, I am just starting to do cryosections and I ran into a problem. I have mouse tissue that we freeze in OCT (we tried different tissue preprocessing: PFA immersion fix, whole animal PFA perfusion, sucrose gradient). I am sectioning skeletal muscle at 10 micron. When I look at the sections directly after sectioning (slide is still cold), the morphology looks ok. However, if I allow the slide to warm to room temp than the tissue start to 'tear' apart and the individual fibers get separated. I could keep the sections at -20C and they will be ok, however, during staining the slides will warm to RT and the above problem occurs.
What can I do to prevent the tissue from starting to tear? Thanks, Gerben, Rotterdam, The Netherlands _________________________________________________________________ Express yourself instantly with MSN Messenger! Download today it's FREE! http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
