Hi-
I am new to using IHC and I need some advice on Antigen Retrieval. I usually do it in an autoclave for 15min at 121C for FFPE human brain tissue (in Citrate or Tris Buffer), but by the end my tissue looks pretty bad and parts have even fallen off. The tissue is fixed on SuperFrost Plus Slides. Any suggestions would be really helpful. Thanks! N. Patten Post-Baccalaureate Fellow/IRTA National Institutes of Health _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet