Carl: In mineral oil all tissues will "clear" as in old days, when we used xylene. This is good test for adequate dehydration and clearing I uses now. Mineral oil very well mixes as with IPA at 50oC, as with paraffin at 60oC or so. Regards, Maxim Peshkov Russia, Taganrog.
Carl Hobbs <[EMAIL PROTECTED]> wrote: > I never use ethanol for any histological-based > system. > I use IMS (Industrial methylated spirit/ Industrial > Denatured Alcohol -IDA ) > Is IMS less/more expensive than IPA? > I use IMS for pwax > processing/rehydrating/dehydrating my pwax sections. > ( us Brits will most likely use 74OP = ~99%: VWR > list price is Ј135 for 25 L. However, every large > Organisation buys it considerably cheaper.) > > Rene's suggestion to use IPA for pwax processing is > a very good alternative: you do not need to use a > "clearing " agent, like xylene. > (Of course, your tissues will not "clear", lol: in > the old days it was an indicator that the tissue was > ready to move on to the next stage....these days we > just rely on Protocols ;-) > > In my experience, IPA does not dissolve paraffin wax > at RT BUT, at the 60C temp. of wax in a tissue > processor/wax oven if processing by hand, IPA and wax > are entirely miscible. mailto:[EMAIL PROTECTED] _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet