Has anyone ever tried to continue a DAB reaction after the slides have been washed and coverslipped? I can't think of a reason why this wouldn't work, since we just wash the slides in PBS and water after the DAB step. We use a vectastain kit for this--would we need to add more of the ABC reagent (after removing the coverslips and washing in PBS) and then do DAB?
Emily -- the velocity of time turns her voice into sugar water http://www.youtube.com/watch?v=jNA6zzoObxg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet