I also do not see any reason why you cannot hydrate the sections again and add the DAB. It should work. René J.
--- On Mon, 10/13/08, Emily Sours <[EMAIL PROTECTED]> wrote: From: Emily Sours <[EMAIL PROTECTED]> Subject: [Histonet] restarting DAB reaction To: histonet@lists.utsouthwestern.edu Date: Monday, October 13, 2008, 1:17 PM Has anyone ever tried to continue a DAB reaction after the slides have been washed and coverslipped? I can't think of a reason why this wouldn't work, since we just wash the slides in PBS and water after the DAB step. We use a vectastain kit for this--would we need to add more of the ABC reagent (after removing the coverslips and washing in PBS) and then do DAB? Emily -- the velocity of time turns her voice into sugar water http://www.youtube.com/watch?v=jNA6zzoObxg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
